Development and validation of HPLC method for analysis of dexamethasone acetate in microemulsions

  • Maria Cristina Cocenza Urban Universidade Estadual Paulista Júlio de Mesquita Filho; Faculdade de Ciências Farmacêuticas de Araraquara; Departamento de Fármacos e Medicamentos
  • Rubiana Mara Mainardes Universidade Estadual do Centro-Oeste; Departamento de Farmácia
  • Maria Palmira Daflon Gremião Universidade Estadual Paulista Júlio de Mesquita Filho; Faculdade de Ciências Farmacêuticas de Araraquara; Departamento de Fármacos e Medicamentos
Keywords: Dexamethasone acetate^i1^sdeterminat, Microemulsions^i1^squantitative analy, High performance liquid chromatography^i1^squantitative analy, Analytical method^i1^svalidat

Abstract

A simple, rapid, accurate and sensitive method was developed for quantitative analysis of dexamethasone acetate in microemulsions using high performance liquid chromatography (HPLC) with UV detection. The chromatography parameters were stainless steel Lichrospher 100 RP-18 column (250 mm x 4 mm i.d., 5 μm particle size), at 30 ± 2 ºC. The isocratic mobile phase was methanol:water (65:35; v/v) at a flow rate of at 1.0 mL.min-1. The determinations were performed using UV-Vis detector set at 239 nm. Samples were prepared with methanol and the volume injected was 20 μL. The analytical curve was linear (r² 0.9995) over a wide concentration range (2.0-30.0 μg.mL-1). The presence of components of the microemulsion did not interfere in the results of the analysis. The method showed adequate precision, with a relative standard deviation (RSD) smaller than 3%. The accuracy was analyzed by adding a standard drug and good recovery values were obtained for all drug concentrations used. The HPLC method developed in this study showed specificity and selectivity with linearity in the working range and good precision and accuracy, making it very suitable for quantification of dexamethasone in microemulsions. The analytical procedure is reliable and offers advantages in terms of speed and low cost of reagents.

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Published
2009-03-01
How to Cite
Urban, M., Mainardes, R., & Gremião, M. (2009). Development and validation of HPLC method for analysis of dexamethasone acetate in microemulsions . Brazilian Journal of Pharmaceutical Sciences (Impresso), 45(1), 87-92. https://doi.org/10.1590/S1984-82502009000100010
Section
Original Papers