HPLC method development and validation for the estimation of axitinibe in rabbit plasma

Authors

  • Achanta Suneetha Hindu College of Pharmacy; Department of Pharmaceutical Analysis
  • Sharmila Donepudi V.V. Institute of Pharmaceutical Sciences; Department of Pharmaceutical Analysis

DOI:

https://doi.org/10.1590/s2175-97902017000300012

Keywords:

High performance liquid chromatography/method validation, Axitinibe/determination, Axitinibe/ kidney cancer treatment, Crizotinibe, Rabbit plasma, Tyrosine kinase inhibitor

Abstract

A rapid, sensitive, and accurate high performance liquid chromatography for the determination of axitinibe (AN) in rabbit plasma is developed using crizotinibe as an internal standard (IS). Axitinibe is a tyrosine kinase inhibitor, used in the treatment of advanced kidney cancer, which works by slowing or stopping the growth of cancer cells. The chromatographic separation was performed on a Waters 2695, Kromosil (150 mm × 4.6 mm, 5 µm) column using a mobile phase containing buffer (pH 4.6) and acetonitrile in the ratio of 65:35 v/v with a flow rate of1 mL/min. The analyte and internal standard were extracted using liquid-liquid extraction with acetonitrile. The elution was detected by photo diode array detector at 320 nm.The total chromatographic runtime is 10.0 min with a retention time for axitinibe and IS of 5.685, and 3.606 min, respectively. The method was validated over a dynamic linear range of 0.002-0.2µg/mL for axitinibe with a correlation coefficient of r2 0.999.

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Published

2017-01-01

Issue

Section

Articles

How to Cite

HPLC method development and validation for the estimation of axitinibe in rabbit plasma. (2017). Brazilian Journal of Pharmaceutical Sciences, 53(3), e00012-. https://doi.org/10.1590/s2175-97902017000300012