Biosynthesis of phospholipases and proteinases using clinical and environmental strains of Cryptococcus neoformans

Authors

  • Pedro Henrique Magalhães Cardoso University of São Paulo
  • Rennan Luiz Oliveira dos Santos University of São Paulo
  • Ana Clara Souza University of São Paulo
  • Francisco de Assis Baroni Federal Rural University of Rio de Janeiro
  • Carina Domaneschi University of São Paulo
  • Claudete Rodrigues Paula PhD in microbiology and teacher of the Postgraduate Program in Oral Diagnosis, School of Dentistry, University of São Paulo. São Paulo - Brazil.

DOI:

https://doi.org/10.11606/issn.2357-8041.clrd.2018.133520

Keywords:

Cryptococcus, Cryptococcus neoformans, Enzymes, Virulence, Virulence Factors

Abstract

Objective: To compare the production of enzymes (proteinases and phospholipases) among the clinical and environmental isolates of Cryptococcus neoformans, because researches with clinical and environmental isolates contribute to the understanding of the mechanisms involved in the control of these infections. Methods: In this research, 78 isolates of C. neoformans from clinical and environmental backgrounds were tested to demonstrate a possible associated phenotype and its virulence. Results: We observed that strains of clinical origin had a higher rate of phospholipase production than those of proteinase. There was no such differentiation among strains of environmental origin. Conclusion: These aspects emphasize the role of phospholipase in cases of C. neoformans infections of clinical origin, and may be a marker to evidence these species.

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Author Biographies

  • Pedro Henrique Magalhães Cardoso, University of São Paulo

    Institute of Biosciences, University of São Paulo, São Paulo, SP,
    Brazil

  • Rennan Luiz Oliveira dos Santos, University of São Paulo

    School of Dentistry, University of São Paulo, São Paulo, SP, Brazil

  • Ana Clara Souza, University of São Paulo
    School of Dentistry, University of São Paulo, São Paulo, SP, Brazil
  • Francisco de Assis Baroni, Federal Rural University of Rio de Janeiro

    Federal Rural University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil

  • Carina Domaneschi, University of São Paulo

    School of Dentistry, University of São Paulo, São Paulo, SP, Brazil

  • Claudete Rodrigues Paula, PhD in microbiology and teacher of the Postgraduate Program in Oral Diagnosis, School of Dentistry, University of São Paulo. São Paulo - Brazil.

    Graduate Program in Buccal Diagnosis, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil

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Published

2018-02-22

Issue

2018

Section

Original Research

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