An experimental model for the transplantation of fetal central nervous system cells to the injured spinal cord in rats

Authors

  • Tarcísio Eloy Pessoa de Barros Filho University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Orthopaedics and Traumatology
  • Reginaldo Perilo de Oliveira University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Orthopaedics and Traumatology
  • Ana Maria Tsanaclis University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Orthopaedics and Traumatology
  • Erika Meirelles Kalil Pessoa de Barros University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Orthopaedics and Traumatology
  • Alexandre Fogaça Cristante University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Orthopaedics and Traumatology
  • Ricardo Moreira Palma University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Orthopaedics and Traumatology
  • Cristian Vilela dos Santos University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Orthopaedics and Traumatology
  • Raphael Martus Marcon University of São Paulo; Faculty of Medicine; Hospital das Clínicas; Department of Orthopaedics and Traumatology

DOI:

https://doi.org/10.1590/S0041-87812002000600003

Keywords:

Spinal cord injury, Fetal cells, Rats

Abstract

INTRODUCTION: Traumatic spinal cord injury is one of the most disabling conditions occurring in man and thus stimulates a strong interest in its histopathological, biochemical, and functional changes, primarily as we search for preventive and therapeutic methods. PURPOSE: To develop an experimental model for transplantation of cells from the fetal rat central nervous system to the site of an injured spinal cord of an adult rat in which the transplanted cells survive and become integrated. This experimental model will facilitate investigations of factors that promote regeneration and functional recovery after spinal cord trauma. MATERIAL AND METHODS: Fifteen adult Wistar rats underwent laminectomy, and an spinal cord lesion was made with microdissection. Fetal spinal cord tissue was then transplanted to the site of the injury. The rats were monitored over a 48-hour period, and then their vertebral column was completely removed for histological analysis. RESULTS: In 60% of transplanted rats, the fetal tissue at the injured site remained viable in the site of the lesion.

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Published

2002-01-01

Issue

Section

Original Articles

How to Cite

An experimental model for the transplantation of fetal central nervous system cells to the injured spinal cord in rats . (2002). Revista Do Hospital Das Clínicas, 57(6), 257-264. https://doi.org/10.1590/S0041-87812002000600003