Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene

Authors

  • Alfredo Carlos S. D. Barros University of São Paulo; Faculty of Medicine; Hospital das Clínicas
  • Elisa Naomi K. Muranaka University of São Paulo; Faculty of Medicine; Hospital das Clínicas
  • Lincon Jo Mori University of São Paulo; Faculty of Medicine; Hospital das Clínicas
  • Christina Helena T. Pelizon University of São Paulo; Faculty of Medicine; Hospital das Clínicas
  • Kyoshi Iriya University of São Paulo; Faculty of Medicine; Hospital das Clínicas
  • Giovana Giocondo University of São Paulo; Faculty of Medicine; Hospital das Clínicas
  • José Aristodemo Pinotti University of São Paulo; Faculty of Medicine; Hospital das Clínicas

DOI:

https://doi.org/10.1590/S0041-87812004000500006

Keywords:

Breast cancer, Animal models, Carcinogens, DMBA-induced tumors, Rat mammary carcinogenesis

Abstract

PURPOSE: To test an experimental model of chemical mammary carcinogenesis induction in rats. METHODS: Twenty young virgin Sprague-Dawley female rats, aged 47 days, received 20 mg of 7,12-dimethylbenz(a)anthracene (DMBA) intragastrically by gavage. Afterwards, at 8 and 13 weeks, their mammary glands were examined. At the end of the experiment, the animals were sacrificed, and the mammary tumors were measured and weighed. Tumor fragments were analyzed using light microscopy. RESULTS: Eight weeks after DMBA injection, 16 rats presented at least 1 breast tumor (80%). After 13 weeks, all of them (100%) developed breast carcinomas that were confirmed by histopathological analysis. CONCLUSION: This experimental animal model of chemical mammary induced carcinogenesis is feasible and can be used in further experiments on the role of tumorigenic biomodulator substances.

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Published

2004-01-01

Issue

Section

Original Research

How to Cite

Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene . (2004). Revista Do Hospital Das Clínicas, 59(5), 257-261. https://doi.org/10.1590/S0041-87812004000500006