Analysis of mitochondrial enzymatic activity in blood lymphomonocyte fractions during infection with different Trypanosoma cruzi strains

Authors

  • Alejandra L. Báez Universidad Nacional de Córdoba, Facultad de Ciencias Médicas https://orcid.org/0000-0002-4682-9979
  • María S. Lo Presti Universidad Nacional de Córdoba, Facultad de Ciencias Médicas
  • Paola C. Bazán Universidad Nacional de Córdoba, Facultad de Ciencias Médicas
  • Mariana Strauss Universidad Nacional de Córdoba, Facultad de Ciencias Médicas
  • Daniela A. Velázquez López Universidad Nacional de Córdoba, Facultad de Ciencias Médicas
  • Noemí Miler Universidad Nacional de Córdoba, Facultad de Ciencias Médicas
  • Héctor W. Rivarola Universidad Nacional de Córdoba, Facultad de Ciencias Médicas
  • Patricia A. Paglini-Oliva Universidad Nacional de Córdoba, Facultad de Ciencias Médicas

DOI:

https://doi.org/10.1590/S1678-9946202062015

Keywords:

Chagas disease, Krebs cycle, Lymphomonocyte fraction, Mitochondria, Respiratory chain

Abstract

Proinflammatory and inflammatory mediators induced by Trypanosoma cruzi infection increase the oxidative stress, generating toxicity for cells targeting mitochondria of different tissues. We studied the activity of citrate synthase and complexes I-IV of respiratory chain in mitochondria of blood lymphomonocyte fraction, from albino Swiss mice infected with different isolates of T. cruzi, during Chagas disease evolution. Complexes I-IV were modified in infected groups (p<0.05) in all the stages, and an inflammatory process of different magnitudes was detected in the heart and skeletal muscle according to the isolate. The citrate synthase activity presented modifications in the SGO Z12 and the Tulahuen group (p<0.05). Hearts showed fiber fragmentation and fibrosis; skeletal muscle presented inflammatory infiltrates and in the Tulahuen infected group, there were also amastigote nests. The inflammatory processes produced an oxidative stress that induced different alterations of mitochondrial enzymes activities in the lymphomonocyte fraction that can be detected by a simple blood extraction, suggesting that they could be used as disease markers, especially in the indeterminate phase of Chagas disease.

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Published

2020-02-11

Issue

Section

Original Articles

How to Cite

Analysis of mitochondrial enzymatic activity in blood lymphomonocyte fractions during infection with different Trypanosoma cruzi strains. (2020). Revista Do Instituto De Medicina Tropical De São Paulo, 62, e15. https://doi.org/10.1590/S1678-9946202062015