Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors

Authors

  • Márcia Oliveira Casotti Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias
  • Ronaldo Cesar Borges Gryschek Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias
  • Fabiana Martins de Paula Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias https://orcid.org/0000-0003-3343-0149
  • Michele Gomes-Gouvêa Universidade de São Paulo, Instituto de Medicina Tropical da São Paulo
  • João Renato Rebello Pinho Universidade de São Paulo, Instituto de Medicina Tropical da São Paulo
  • Roseli Tuan Superintendência de Controle de Endemias
  • Emmanuel Dias-Neto Hospital A. C. Camargo, Centro Internacional de Pesquisas e Ensino
  • Expedito de Albuquerque Luna Universidade de São Paulo, Faculdade de Medicina
  • Maria Cristina Carvalho do Espírito-Santo Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias

DOI:

https://doi.org/10.1590/S1678-9946202062017

Keywords:

Schistosoma mansoni, Biomphalaria glabrata, Molecular diagnosis, Surveillance, Snail, Vector, molecular diagnosis, Polymerase Chain Reaction

Abstract

Approximately 240 million people worldwide are infected by Schistosoma. In Brazil, one of the main intermediate hosts of this parasite is Biomphalaria glabrata snails. The early detection of larval stages in intermediate hosts is an important challenge to public health, but it also represents an opportunity as a new alternative to indicate earlier natural infections before cercariae differentiation and emergence. In this context, we demonstrated that PCR amplification of a 28S gene fragment from the parasite does demonstrate S. mansoni infection in snails 14 days post infection. This conventional polymerase chain reaction amplified clear bands and was able to detect parasitic infection in the intermediate host B. glabrata under experimental conditions. However, we reinforce that this approach requires deeper investigations and further comparisons to confirm its specificity and sensitivity in earlier time points after miracidia infection. This approach has relevant potential as an effective molecular-based strategy for the monitoring of schistosomiasis transmission.

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Published

2020-02-28

Issue

Section

Original Articles

How to Cite

Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors. (2020). Revista Do Instituto De Medicina Tropical De São Paulo, 62, e17. https://doi.org/10.1590/S1678-9946202062017