Growth promotion of common bean and genetic diversity of bacteria from Amazon pastureland

Authors

  • Linnajara de Vasconcelos Martins Ferreira Federal Institute of Pará; Campus Marabá Rural
  • Fernanda de Carvalho Federal University of Lavras; Dept. of Soil Science – Sector of Biology, Microbiology and Biological Processes
  • Júlia Fonseca Colombo Andrade Federal University of Lavras; Dept. of Soil Science – Sector of Biology, Microbiology and Biological Processes
  • Fatima Maria de Souza Moreira Federal University of Lavras; Dept. of Soil Science – Sector of Biology, Microbiology and Biological Processes

DOI:

https://doi.org/10.1590/1678-992x-2017-0049

Keywords:

Phaseolus vulgaris, co-inoculation, endophytic bacteria, rhizobia

Abstract

A significant number of bacterial species, particularly in the rhizosphere, may benefit plant growth and development. This group of bacteria is known as plant growth-promoting rhizobacteria (PGPR). This study identified genetically isolates of common bean nodules used to trap bacteria from Amazon pastureland and investigated their capacity of nodulating and promoting growth of common bean when inoculated or co-inoculated with CIAT899 strain (Rhizobium tropici). Two experiments were carried out in a greenhouse, in axenic conditions, using the common bean cultivar Talismã. In the first experiment, 56 PGPR strains were evaluated individually regarding growth promotion and nodulation. In the second experiment, inoculation of seven PGPR strains previously selected in the first experiment was evaluated in three forms of N supply: Co-inoculation with CIAT 899 in the presence of low N-mineral concentration; individual inoculation in the presence of high N-mineral concentration; and individual inoculation in the presence of low N-mineral concentration. The 16S rRNA gene sequencing showed predominance of Pseudomonas genus, identified in 35 % of the sequenced strains. Other genera were identified: Rhizobium, Burkholderia, Xanthomonas and Bacillus. Inoculation of the seven strains with CIAT 899 promoted distinct plant growth in different forms of N supply. In addition, N-mineral supply can be replaced by co-inoculation with strains of Pseudomonas sp. (UFLA 02-281 and UFLA 02-293) and Bacillus sp. (UFLA 02-298) identified in this study.

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Published

2018-12-01

How to Cite

Ferreira, L. de V. M., Carvalho, F. de, Andrade, J. F. C., & Moreira, F. M. de S. (2018). Growth promotion of common bean and genetic diversity of bacteria from Amazon pastureland. Scientia Agricola, 75(6), 461-469. https://doi.org/10.1590/1678-992x-2017-0049

Issue

Section

Agricultural Microbiology