Partial purification and properties of ornithine transcarbamylase from bean leaves (Phaseolus vulgaris L.)

Authors

  • L.E. Gutierrez Universidade de São Paulo; E.S.A.Luiz de Queiroz; Departamento de Química
  • O.J. Crocomo Universidade de São Paulo; E.S.A.Luiz de Queiroz; Departamento de Química

DOI:

https://doi.org/10.1590/S0071-12761989000100008

Keywords:

ornithine transcarbamylase, bean, Phaseolus vulgaris

Abstract

Ornithine transcarbamylase (E.C. 2.1.3.3) was extracted from leaves of bean plants (Phaseolus vulgaris L. cv. carioca) and partially purified by ammonium sulfate precipitation at pH 7,2 and 7.8. The enzyme preparation showed an optimum pH of 7.8 and Km of 3.18mM for ornithine and of 3.93mM for carbamil-phosphate.

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Published

1989-01-01

Issue

Vol. 46 No. 1 (1989)

Section

Articles

How to Cite

Gutierrez, L., & Crocomo, O. (1989). Partial purification and properties of ornithine transcarbamylase from bean leaves (Phaseolus vulgaris L.) . Anais Da Escola Superior De Agricultura Luiz De Queiroz, 46(1), 111-123. https://doi.org/10.1590/S0071-12761989000100008