Selective photodynamic effects on cervical adenocarcinoma cells provided by F127 Pluronic®-based micelles modulating hypericin delivery

Authors

  • Kayane Harumi Mashiba Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá, Maringá, Paraná, Brazil
  • Lucimara Rofrigues Carobeli Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá, Maringá, Paraná, Brazil https://orcid.org/0000-0003-3066-4708
  • Maria Vítoria Felipe de Souza Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá, Maringá, Paraná, Brazil
  • Lyvia Eloiza de Freitas Meirelles Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá, Maringá, Paraná, Brazil
  • Natália Lourenço Mari Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá, Maringá, Paraná, Brazil
  • Gabriel Batista César Department of Chemistry, Universidade Estadual de Maringá, Maringá, Paraná, Brazil
  • Renato Sonchini Gonçalves Department of Chemistry, Universidade Estadual de Maringá, Maringá, Paraná, Brazil
  • Wilker Caetano Department of Chemistry, Universidade Estadual de Maringá, Maringá, Paraná, Brazil
  • Edilson Damke Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá, Maringá, Paraná, Brazil
  • Vânia Ramos Sela da Silva Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá, Maringá, Paraná, Brazil
  • Gabrielle Marconi Zago Ferreira Damke Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá, Maringá, Paraná, Brazil
  • Marcia Edilaine Lopes Consolaro Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá, Maringá, Paraná, Brazil https://orcid.org/0000-0001-9102-4865

DOI:

https://doi.org/10.1590/s2175-97902023e22459

Keywords:

Photochemotherapy, Endocervical adenocarcinoma, HeLa cells, Hypericin, Pluronic F127, Drug delivery systems

Abstract

Cervical cancer is a leading cause of death among women. The endocervical adenocarcinoma (ECA) represents an aggressive and metastatic type of cancer with no effective treatment options currently available. We evaluated the antitumoral and anti-migratory effects of hypericin (HYP) encapsulated on Pluronic F127 (F127/HYP) photodynamic therapy (PDT) against a human cell line derived from invasive cervical adenocarcinoma (HeLa) compared to a human epithelial cell line (HaCaT). The phototoxicity and cytotoxicity of F127/HYP were evaluated by the following assays: colorimetric assay, MTT, cellular morphological changes by microscopy and long-term cytotoxicity by clonogenic assay. In addition, we performed fluorescence microscopy to analyze cell uptake and subcellular distribution of F127/HYP, cell death pathway and reactive oxygen species (ROS) production. The PDT mechanism was determined with sodium azide and D-mannitol and cell migration by wound-healing assay. The treatment with F127/HYP promoted a phototoxic result in the HeLa cells in a dose-dependent and selective form. Internalization of F127/HYP was observed mainly in the mitochondria, causing cell death by necrosis and ROS production especially by the type II PDT mechanism. Furthermore, F127/HYP reduced the long-term proliferation and migration capacity of HeLa cells. Overall, our results indicate a potentially application of F127/HYP micelles as a novel approach for PDT with HYP delivery to more specifically treat ECA.

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Published

2023-05-22

Issue

Vol. 59 (2023)

Section

Original Article

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How to Cite

Selective photodynamic effects on cervical adenocarcinoma cells provided by F127 Pluronic®-based micelles modulating hypericin delivery. (2023). Brazilian Journal of Pharmaceutical Sciences, 59, e22459. https://doi.org/10.1590/s2175-97902023e22459