Overexpression of lncRNA-MEG3 inhibits endometrial cell proliferation and invasion via miR-21–5p/DNMT3B/Twist

Authors

  • Shaoyan Yang The Second Affiliated Hospital of Kunming Medical University
  • Limei Feng The Second Affiliated Hospital of Kunming Medical University
  • Qin Zhang The Second Affiliated Hospital of Kunming Medical University
  • Lu Wu The Second Affiliated Hospital of Kunming Medical University
  • Qinghua Zhao The Second Affiliated Hospital of Kunming Medical University
  • Youfang Hou The Second Affiliated Hospital of Kunming Medical University
  • Bo Yan The Second Affiliated Hospital of Kunming Medical University
  • Suxian Zhang The Second Affiliated Hospital of Kunming Medical University https://orcid.org/0009-0004-1196-9391

DOI:

https://doi.org/10.1016/j.clinsp.2023.100235

Keywords:

lncRNA-MEG3, miR-21–5p, DNMT3B, TWIST, hESCs

Abstract

Recent studies have found that lncRNA-MEG3(MEG3) plays an important role in the development of EMs (Endometriosis), but the specific mechanism needs to be further explored. This study aimed to investigate the effect of MEG3 on the proliferation, invasion of EMs cells. The authors used RT-qPCR to detect the expression of MEG3 and miR-21–5p in EMs tissues and hESCs cells, MTT and Transwell to detect cell proliferation and invasion, western blotting assay to detect the expression of DNMT3B and Twist, MSP to detect the methylation of Twist. The present study's detection results showed that MEG3 was lowly expressed in EMs tissues and hESCs cells, and overexpression of MEG3 could down-regulate miR-21–5p and inhibit endometrial cell proliferation and invasion. In addition, overexpression of MEG3 upregulated the expression of DNMT3B and promoted the methylation of TWIST. In conclusion, the present findings suggest that MEG3 is downregulated in EMs tissues, and overexpression of MEG3 can promote the activity of DNA methyltransferase DNMT3B by downregulating miR-21–5p, thereby promoting the methylation of Twist, downregulating Twist level to inhibits hESCs cells proliferation and invasion.

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Published

2023-06-29

Issue

Section

Original Articles

How to Cite

Overexpression of lncRNA-MEG3 inhibits endometrial cell proliferation and invasion via miR-21–5p/DNMT3B/Twist. (2023). Clinics, 78, 100235. https://doi.org/10.1016/j.clinsp.2023.100235