Effect of high-voltage electrical stimulation and topical insulin on experimental cutaneous lesions
DOI:
https://doi.org/10.1590/1809-2950/15311923042016Keywords:
Electric Stimulation, Topical Insulin, Wistar Rats/LesionsAbstract
This study aims to evaluate the effect of cathodic high voltage electrical stimulation (HVES), associated with topical insulin, on rat integumentary lesions. For this purpose, 42 Wistar rats (240±30 g) were submitted to surgical removal of 1 cm2 of dorsal skin and divided into six groups (n=7), treated for seven consecutive days: Control (C), placebo electrical stimulation (PES), cathodic electrical stimulation (ES), topical insulin (TI), placebo insulin (PI) and HVES associated with topical insulin (ES+I). HVES was administered 24 hours after surgery, 30 minutes per day, with a frequency of 100 Hz and a mean voltage of 60 V, maintained at the motor threshold. Lesion areas were recorded macroscopically on the first, fourth and eighth day, submitted to histological treatment for inclusion in paraplast® and staining in Hematoxylin and Eosin. Epithelialization and the numerical profile of the cells were obtained by histometric analysis. The Shapiro-Wilk and Anova one-way test was followed by the Bonferroni (p<0.05). There was a significant reduction in the area of the lesion by the eighth day of treatment, both in the ES and ES+I groups, when compared with other groups. Reepithelialization did not differ between groups, but the distance between the edges of the lesion was lower in the ES and ES+I groups. These same groups showed a significant increase (p<0.05) in the number of fibroblasts and a decrease in leukocytes. Thus, we can conclude that cathodic HVES accelerated the lesion repair process, with the topical application of insulin showing no additional effect.Downloads
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