Resistance jump training may reverse the weakened biomechanical behavior of tendons of diabetic Wistar rats
DOI:
https://doi.org/10.1590/1809-2950/17198024042017Keywords:
Diabetes Mellitus, Resistance Training, Connective Tissue, Animal ModelAbstract
Background: resistance training is widely applied in non-diabetic physical protocol showing effectiveness in improving the tendon tissue. To address this gap, we assessed the effects of resistance training on aquatic environment, on the biomechanical properties of the calcaneal tendon of diabetic Wistar rats. Methods: 59 male Wistar rats were evaluated for 60 days, they were randomly divided into the following groups: Sedentary Control Group (SCG, n=15), Sedentary Diabetic Group (SDG, n=15), Trained Control Group (TCG, n=14) and Trained Diabetic Group (TDG, n=15). After randomization the animals from the SDG and the TDG were induced to Diabetes Mellitus by intraperitoneal injection of Streptozotocin (60 mg/kg). The animals on the trained groups performed resistance exercise that consisted of jumping in an aquatic environment. After nine weeks the calcaneal tendons were collected and tractioned on a conventional mechanical testing machine. Results: the analysis of biomechanical parameters showed lower values in elastic modulus (p=0.000), maximum strength tension (p=0.000) and energy/area (p=0.008) in TDG compared to SDG in addition to an increase on the cross-sectional area (p=0.002). There was no difference for the specific deformation variable. Conclusion: the training protocol used restored some biomechanical parameters of the calcaneal tendon in rats induced to diabetes, thus, resulting in an improvement of its mechanical efficiency.Downloads
References
Akturk M, Karaahmetoglu S, Kacar M, Muftuoglu O. Thickness
of the supraspinatus and biceps tendons in diabetic patients.
Diabetes Care. 2002;25(2):408. doi: 10.2337/diacare.25.2.408
Oliveira RR, Mayworn SH, Oliveira MC. Existe relação da
microangiopatia diabética com as alterações estruturais dos
tendões? Diabetes Clín. 2003;7(6):446-9.
Bolton NR, Smith KE, Pilgram TK, Mueller MJ, Bae KT.
Computed tomography to visualize and quantify the plantar
aponeurosis and flexor hallucis longus tendon in the diabetic
foot. Clin Biomech (Bristol, Avon). 2005;20(5):540-6. doi:
1016/j.clinbiomech.2004.12.007
Akturk M, Ozdemir A, Maral I, Yetkin I, Arslan M. Evaluation
of Achilles tendon thickening in type 2 diabetes mellitus.
Exp Clin Endocrinol Diabetes. 2007;115(2):92-6. doi:
1055/s-2007-955097
Grant WP, Sullivan R, Sonenshine DE, Adam M, Slusser JH,
Carson KA, Vinik AI. Electron microscopic investigation of
the effects of diabetes mellitus on the Achilles tendon. J Foot
Ankle Surg. 1997;36(4):272-8.
Aydeniz A, Gursoy S, Guney E. Which musculoskeletal
complications are most frequently seen in type 2
diabetes mellitus? J Int Med Res. 2008;36(3):505-11. doi:
1177/147323000803600315
De Oliveira RR, Lemos A, de Castro Silveira PV, da Silva RJ, de
Moraes SR. Alterations of tendons in patients with diabetes
mellitus: a systematic review. Diabet Med. 2011;28(8):886-95.
doi: 10.1111/j.1464-5491.2010.03197.x
Reddy GK, Stehno-Bittel L, Enwemeka CS. Glycation-induced
matrix stability in the rabbit achilles tendon. Arch Biochem
Biophys. 2002;399(2):174-80. doi: 10.1006/abbi.2001.2747
Fox AJ, Bedi A, Deng XH, Ying L, Harris PE, Warren RF, Rodeo
SA. Diabetes mellitus alters the mechanical properties of the
native tendon in an experimental rat model. J Orthop Res.
;29(6):880-5. doi: 10.1002/jor.21327
De Oliveira RR , Lira KD , Silveira PV, Coutinho MP, Medeiros MN
, Teixeira MF, Moraes SRA. Mechanical properties of achilles
tendon in rats induced to experimental diabetes. Ann Biomed
Eng. 2011;39(5):1528-34. doi: 10.1007/s10439-011-0247-z
Luciano E, Mello MAR. Atividade física e metabolismo de
proteínas em músculo de ratos diabéticos experimentais.
Rev Paul Educ Fís. 1998;12(2):202-9.
Oliveira CAM, Rogatto GP, Luciano E. Efeitos do treinamento
físico de alta intensidade sobre os leucócitos de ratos
diabéticos. Rev Bras Med Esp. 2002;8(6):219-24. doi: 10.1590/
S1517-86922002000600003
Kemmer FW, Berger M. The diabetic patient and sports:
references and guidelines for daily practice. Ther Umsch.
;40(10):875-82.
Huang HH, Farmer K, Windscheffel J, Yost K, Power M, Wright
DE, Stehno-Bittel L. Exercise increases insulin content and
basal secretion in pancreatic islets in type 1 diabetic mice.
Exp Diabetes Res. 2011;2011:481427. doi: 10.1155/2011/481427
Curwin SL, Vailas AC, Wood J. Immature tendon adaptation
to strenuous exercise. J Appl Physiol. 1988;65(5):2297-301.
Shadwick RE. Elastic energy storage in tendons: mechanical
differences related to function and age. J Appl Physiol.
;68(3):1033-40.
Simonsen EB, Klitgaard H, Bojsen-Moller F. The influence of
strength training, swim training and ageing on the Achilles
tendon and m. soleus of the rat. J Sports Sci. 1995;13(4):291-
doiu: 10.1080/02640419508732242
Buchanan CI, Marsh RL. Effects of long-term exercise on the
biomechanical properties of the Achilles tendon of guinea
fowl. J Appl Physiol. 2001;90(1):164-71.
Bezerra MA, Lemos A, Lira KD, Silveira PV, Coutinho
MP, Moraes SR. Does aerobic exercise training promote
changes in structural and biomechanical properties of the
tendons in experimental animals? A systematic review. Biol
Sport. 2012;29(4):249-54. doi: 10.5604/20831862
Bezerra MA, Santos de Lira KD, Coutinho MP, de Mesquita
GN, Novaes KA, da Silva RT, de Brito Nascimento AK, Inácio
Teixeira MF, Moraes SR. Biomechanical and structural
parameters of tendons in rats subjected to swimming
exercise. Int J Sports Med. 2013;34(12):1070-3. doi:
1055/s-0033-1345130
Oliveira RR, Bezerra MA, de Lira KD, Novaes KA, Teixeira MF,
Chaves CC, Moraes SR. Aerobic physical training restores
biomechanical properties of Achilles tendon in rats chemically
induced to diabetes mellitus. J Diabetes Complications.
;26(3):163-8. doi: 10.1016/j.jdiacomp.2012.03.017
Biewener AA, Roberts TJ. Muscle and tendon contributions
to force, work, and elastic energy savings: a comparative
perspective. Rev Exerc Sport Sci. 2000;8(3):99-107.
Hof AL, Van Zandwijk JP, Bobbert MF. Mechanics of
human triceps surae muscle in walking, running and
jumping. Acta Physiol Scand. 2002;174(1):17-30. doi:
1046/j.1365-201x.2002.00917.x
Roberts TJ, Marsh RL. Probing the limits to muscle-powered
accelerations: lessons from jumping bullfrogs. J Exp Biol.
;206:2567-80. doi: 10.1242/jeb.00452
Dall’Ago P, Silva VO, De Angelis KL, Irigoyen MC, Fazan RJr,
Salgado HC. Reflex control of arterial pressure and heart rate
in short-term streptozotocin diabetic rats. Braz J Med Biol Res.
;35(7):843-9. doi: 10.1590/S0100-879X2002000700013
Carvalho EN, Carvalho NAS, Ferreira LM. Experimental
model of induction of diabetes mellitus in rats.
Acta Cir Bras. 2003;18(no. spe):60-4. doi: 10.1590/
S0102-86502003001100009
Rogatto GP, Luciano E. Efeitos do treinamento físico intenso
sobre o metabolismo de carboidratos. Rev Bras Ativ Fís
Saúde. 2001;6(2):39-46.
Reddy GK. Cross-linking in collagen by nonenzymatic
glycation increases the matrix stiffness in rabbit Achilles
tendon. Exp Diabesity Res. 2004;5(2):143-53. doi:
1080/15438600490277860
Bezerra MA, Nery CS, Silveira PVC, Mesquita GN, Figueiredo
TG, Teixeira MFHBI, Moraes SRA. Previous physical exercise
slow down the complications from experimental diabetes
in the calcaneal tendon. Muscles Ligaments Tendons J.
;6(1):97-103. doi: 10.11138/mltj/2016.6.1.097
Papanas N, Courcoutsakis N, Papatheodorou K,
Daskalogiannakis G, Maltezos E, Prassopoulos P. Achilles
tendon volume in type 2 diabetic patients with or without
peripheral neuropathy: MRI study. Exp Clin Endocrinol
Diabetes. 2009;117(10): 645-8. doi: 10.1055/s-0029-1224121
Kjaer M, Langberg H, Miller BF, Boushel R, Crameri R, Koskinen
S, et at. Metabolic activity and collagen turnover in human
tendon in response to physical activity. J Musculoskelet
Neuronal Interact. 2005;5(1):41-52.
Pauli JR, Cintra DE, Souza CT, Ropelle ER. New mechanisms
by which physical exercise improves insulin resistance in the
skeletal muscle. Arq Bras Endocrinol Metab. 2009;53(4):399-
doi: 10.1590/S0004-27302009000400003
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