Preclinical study of experimental burns treated with photobiomodulation and Human Amniotic Membrane, both isolated and associated

Authors

DOI:

https://doi.org/10.1590/1518-8345.5552.3727

Keywords:

Burns; Amnion; Low-Level Light Therapy; Wound Healing; Skin; Rats.

Abstract

Objective: to evaluate the effect of photobiomodulation with low-level 660 nm laser alone or associated with Human Amniotic Membrane in the repair of partial-thickness burns in rats. Method: an experimental study conducted with 48 male Wistar rats, randomized into four groups: Control, Human Amniotic Membrane, Low-Level Laser Therapy, and Low-Level Laser Therapy associated with Human Amniotic Membrane. The histopathological characteristics of the skin samples were analyzed 7 and 14 days after the burn. The data obtained were submitted to the Kolmogorov-Smirnov and Mann-Whitney tests. Results: the histological analysis of the burn injuries showed a decrease in inflammation (p<0.0001) and an increase in proliferation of fibroblasts (p<0.0001) mainly at 7 days in all treatments related to the control group. At 14 days, the greater effectiveness in accelerating the healing process was significant (p<0.0001) in the Low-Level Laser Therapy group associated with the Human Amniotic Membrane. Conclusion: the association of photobiomodulation therapies with the Human Amniotic Membrane allowed verifying a reduction in the healing process time of the experimental lesions, stimulating its proposal as a treatment protocol in partial-thickness burns.

Downloads

Download data is not yet available.

Downloads

Published

2023-03-06

Issue

Vol. 31 (2023)

Section

Original Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

RLAE’s authorship concept is based on the substantial contribution by each of the individuals listed as authors, mainly in terms of conceiving and planning the research project, collecting or analyzing and interpreting data, writing and critical review. Indication of authors’ names under the article title is limited to six. If more, authors are listed on the online submission form under Acknowledgements. The possibility of including more than six authors will only be examined on multicenter studies, considering the explanations presented by the authors.
Including names of authors whose contribution does not fit into the above criteria cannot be justified. Those names can be included in the Acknowledgements section.
 Authors are fully responsible for the concepts disseminated in their manuscripts, which do not necessarily reflect the editors’ and editorial board’s opinion.

 

How to Cite

Preclinical study of experimental burns treated with photobiomodulation and Human Amniotic Membrane, both isolated and associated. (2023). Revista Latino-Americana De Enfermagem, 31, e3727. https://doi.org/10.1590/1518-8345.5552.3727