Efeito do diluente à base de gema de ovo e remoção do plasma seminal na viabilidade espermática do sêmen refrigerado de jumento
DOI:
https://doi.org/10.11606/issn.1678-4456.bjvras.2021.174301Palavras-chave:
Jumento, Sêmen resfriado, Preservação de sêmen, Plasma seminal, Diluente de gema de ovoResumo
O desenvolvimento de protocolos de sêmen resfriado eficazes é essencial para aumentar as taxas de prenhez e eficiência reprodutiva em jumentos. O objetivo desse estudo foi avaliar o efeito do diluente à base de proteínas do leite com 1 ou 2% de gema de ovo sobre os parâmetros cinéticos do sêmen e integridade da membrana em sêmen resfriado de jumento, com ou sem a remoção do plasma seminal. Vinte e quatro ejaculados de seis jumentos foram coletados. Cada ejaculado foi dividido em quatro alíquotas e diluído em diluente com 1% (EY1) ou 2% (EY2) de gema de ovo. Uma amostra por grupo foi centrifugada e o plasma seminal removido (grupos CEY1 e CEY2, respectivamente). Os pellets foram novamente ressuspendidos nas mesmas concentrações e diluentes. Em seguida, as quatro alíquotas foram refrigeradas a 5°C por 24 horas. Amostras de sêmen fresco e refrigerado foram avaliadas quanto à motilidade espermática e integridade da membrana plasmática. Motilidade total, motilidade progressiva, parâmetros de cinética espermática ou células espermáticas vivas não apresentaram diferença significativa quando o sêmen foi resfriado com diluente suplementado com 1% ou 2% de gema de ovo. A remoção do plasma seminal não afetou a motilidade total ou os parâmetros de cinética espermática; entretanto, a motilidade progressiva diminuiu (P<0,05) quando o sêmen foi diluído com 2% de gema de ovo e o plasma seminal removido. Nas amostras centrifugadas, a integridade da membrana foi afetada (P<0,05). Em conclusão, os resultados sugerem que não há diferença na cinética espermática e na integridade da membrana quando 1% ou 2% de gema de ovo são adicionados ao diluente Equiplus(R) e a remoção do plasma seminal por centrifugação não teve nenhum efeito benéfico no resfriamento de sêmen de jumento. Mais estudos são necessários para relacionar esses resultados com testes de fertilidade in vivo com sêmen resfriado em jumentos.
Downloads
Referências
Ali SS, Marcondes MCG, Bajova H, Dugan LL, Conti B. Metabolic depression and increased reactive oxygen species production by isolated mitochondria at moderately lower temperatures. Journal of Biological Chemistry. 2010;285(42):32522-8. https://doi.org/10.1074/jbc.M110.155432. PMid:20716522.
Alonso C, Castañeira C, Castex CB, Ferrante A, Arraztoa C, Flores A, Gambarotta M, Miragaya M, Losinno L. Effects of extenders and centrifugation on viability of donkey cooled semen. Preliminary Results. Proceedings of the 1st International Symposium on Donkey Science. 2017. Aug 14-17; Shangai China: Dong’e E Jiao Co, Shandong. 196–200.
Alvarez AL, Serres C, Crespo F, Santiago I, Mateos EG-CC. Natural service and artificial insemination with cooled semen of Zamorano-Leones donkey. Proceedings of the 15th International Congress Animal Reproduction; 2004 Aug 8-12; Porto Seguro, 2004.
Aurich C. Factors affecting the plasma membrane function of cooled-stored stallion spermatozoa. Anim. Reprod. Sci. 2005;89(1-4 Spec Issue):65-75.
Batellier F, Duchamp G, Vidament M, Arnaud G, Palmer E, Magistrini M. Delayed insemination is successful with a new extender for storing fresh equine semen at 15 °C under aerobic conditions. Theriogenology. 1998;50(2):229-36. https://doi.org/10.1016/S0093-691X(98)00130-7. PMid:10734490.
Batellier F, Magistrini M, Fauquant J, Palmer E. Effect of milk fractions on survival of equine spermatozoa. Theriogenology. 1997;48(3):391-410. https://doi.org/10.1016/S0093-691X(97)00250-1. PMid:16728137.
Batellier F, Vidament M, Fauquant J, Duchamp G, Arnaud G, Yvon JM, Magistrini M. Advances in cooled semen technology. Anim Reprod Sci. 2001;68(3–4):181-90. https://doi.org/10.1016/S0378-4320(01)00155-5. PMid:11744263.
Bergeron A, Crête M-H, Brindle Y, Manjunath P. Low-density lipoprotein fraction from Hen’s egg yolk decreases the binding of the major proteins of bovine seminal plasma to sperm and prevents lipid efflux from the Sperm Membrane 1. Biol Reprod. 2004;70(3):708-17. https://doi.org/10.1095/biolreprod.103.022996. PMid:14613896.
Bergeron A, Manjunath P. New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Mol Reprod Dev. 2006;73(10):1338-44. https://doi.org/10.1002/mrd.20565. PMid:16868925.
Bergeron A, Villemure M, Lazure C, Manjunath P. Isolation and characterization of the major proteins of ram seminal plasma. Mol Reprod Dev. 2005;71(4):461-70. https://doi.org/10.1002/mrd.20310. PMid:15892046.
Calvete JJ, Nessau S, Mann K, Sanz L, Sieme H, Klug E, Töpfer-Petersen E. Isolation and biochemical characterization of stallion seminal-plasma proteins. Reprod Domest Anim. 1994;29(5):411-26. https://doi.org/10.1111/j.1439-0531.1994.tb00588.x.
Camillo F, Rota A, Biagini L, Tesi M, Fanelli D, Panzani D. The current situation and trend of donkey industry in Europe. J Equine Vet Sci. 2018;65:44-9. https://doi.org/10.1016/j.jevs.2017.11.008.
Carvalho LE, Silva Filho JM, Palhares MS, Sales ALR, Gonczarowska AT, Oliveira HN, Resende MC, Neves MG, Madison RJ. Physical characteristics and fertility of fractionated donkey semen cooled at 5 °C. Arq Bras Med Vet Zootec. 2017;69(1):29-38. https://doi.org/10.1590/1678-4162-7976.
Choong C, Wales R. The Effect of Cold Shock on Spermatozoa. Aust J Biol Sci. 1962;15(3):543-51. https://doi.org/10.1071/BI9620543.
Cochran JD, Amann RP, Froman DP, Pickett BW. Effects of centrifugation, glycerol level, cooling to 5 degrees C, freezing rate and thawing rate on the post-thaw motility of equine sperm. Theriogenology. 1984;22(1):25-38. https://doi.org/10.1016/0093-691X(84)90470-9 PMid:16725933.
Cottorello ACP, Amancio RC, Henry M, Borges I. Effect of storage temperature and extenders on “in vitro” activity of donkey spermatozoa. Theriogenology. 2002;88:325-8.
De Leeuw FE, De Leeuw AM, Den Daas JHG, Colenbrander B, Verkleij AJ. Effects of various cryoprotective agents and membrane-stabilizing compounds on bull sperm membrane integrity after cooling and freezing. Cryobiology. 1993;30(1):32-44. https://doi.org/10.1006/cryo.1993.1005. PMid:8440128.
Di Palma T, Cecchini S, MacChia G, Pasolini MP, Cocchia N, Boni R. Kinematic, bioenergetic and oxidative evaluations of donkey sperm preserved at +4°C. Zygote. 2020;28(4):300-7. https://doi.org/10.1017/S096719942000012X. PMid:32285764.
Dini P, Troch L, Lemahieu I, Deblende P, Daels P. Validation of a portable device (iSperm) for the assessment of stallion sperm motility and concentration. Reprod Domest Anim. 2019;54(8):1113-20. https://doi.org/10.1111/rda.13487. PMid:31177582.
Jasko DJ, Hathaway JA, Schaltenbrand VL, Simper WD, Squires EL. Effect of seminal plasma and egg yolk on motion characteristics of cooled stallion spermatozoa. Theriogenology. 1992;37(6):1241-52. https://doi.org/10.1016/0093-691X(92)90180-Y.
Leboeuf B, Guillouet P, Batellier F, Bernelas D, Bonné JL, Forgerit Y, Renaud G, Magistrini M. Effect of native phosphocaseinate on the in vitro preservation of fresh semen. Theriogenology. 2003;60(5):867-77. https://doi.org/10.1016/S0093-691X(03)00095-5. PMid:12935864.
Martin ICA. Diluents for the preservation of ram spermatozoa 1. Aust J Biol Sci. 1966;19(4):645-53. PMid:5970061.
Mello SLV, Henry M, Souza MC, Oliveira SMP. Effect of split ejaculation and seminal extenders on longevity of donkey semen preserved at 5°C. Arq Bras Med Vet Zootec. 2000;52(4):372-8. https://doi.org/10.1590/S0102-09352000000400015.
Miró J, Taberner E, Rivera M, Peña A, Medrano A, Rigau T, Peñalba A. Effects of dilution and centrifugation on the survival of spermatozoa and the structure of motile sperm cell subpopulations in refrigerated Catalonian donkey semen. Theriogenology. 2009;72(8):1017-22. https://doi.org/10.1016/j.theriogenology.2009.06.012. PMid:19747718.
Newcombe J, Cuervo-Arango J. The effect of time of insemination with fresh cooled transported semen and natural mating relative to ovulation on pregnancy and embryo loss rates in the mare. Reprod Domest Anim. 2011;46(4):678-81. https://doi.org/10.1111/j.1439-0531.2010.01728.x. PMid:21114795.
Pagl R, Aurich JE, Müller-Schlösser F, Kankofer M, Aurich C. Comparison of an extender containing defined milk protein fractions with a skim milk-based extender for storage of equine semen at 5°C. Theriogenology. 2006;66(5):1115-22. https://doi.org/10.1016/j.theriogenology.2006.03.006. PMid:16620943.
Phillips PH, Lardy HA. A yolk-buffer pabulum for the preservation of bull semen. J Dairy Sci. 1940;23(5):399-404. https://doi.org/10.3168/jds.S0022-0302(40)95541-2.
R Core Team. (2018) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna. https://www.R-project.org.
Rota A, Bastianacci V, Magelli C, Panzani D, Camillo F. Evaluation of plasma membrane integrity of donkey spermatozoa. Reprod Domest Anim. 2010;45(2):228-32. https://doi.org/10.1111/j.1439-0531.2008.01241.x. PMid:19144042.
Rota A, Furzi C, Panzani D, Camillo F. Studies on motility and fertility of cooled stallion spermatozoa. Reprod Domest Anim. 2004;39(2):103-9. https://doi.org/10.1111/j.1439-0531.2004.00488.x. PMid:15065992.
Rota A, Magelli C, Panzani D, Camillo F. Effect of extender, centrifugation and removal of seminal plasma on cooled-preserved Amiata donkey spermatozoa. Theriogenology. 2008;69(2):176-85. https://doi.org/10.1016/j.theriogenology.2007.09.003. PMid:17945340.
Rota A, Perulli G, Sabatini C, Panzani D, Camillo F. Cooled semen artificial insemination in donkeys: field results. Reprod Domest Anim. 2017;52:127.
Serres C, Rodriguez A, Alvarez AL, Santiago I, Gabriel J, Gómez-Cuétara C, Mateos E. Effect of centrifugation and temperature on the motility and plasma membrane integrity of Zamorano-Leonés donkey semen. Theriogenology. 2002;58(2–4):329-32.
Talluri TR, Mal G, Ravi SK. Biochemical components of seminal plasma and their correlation to the fresh seminal characteristics in Marwari stallions and Poitou jacks. Vet World. 2017;10(2):214-20. https://doi.org/10.14202/vetworld.2017.214-220. PMid:28344405.
Varner DD, Blanchard TL, Meyers PJ, Meyers SA. Fertilizing capacity of equine spermatozoa stored for 24 hours at 5 or 20°C. Theriogenology. 1989;32(4):515-25. https://doi.org/10.1016/0093-691X(89)90273-2. PMid:16726699.
Villemure M, Lazure C, Manjunath P. Isolation and characterization of gelatin-binding proteins from goat seminal plasma. Reprod Biol Endocrinol. 2003;1(1):1-10. https://doi.org/10.1186/1477-7827-1-39. PMid:12737634.
Vyvial M, Horáčková E, Sedlinská M, Jánová E, Krisová Š, Mráčková M. Determination of selected components in seminal plasma of donkey stallions and their correlation to semen quality parameters Seminal plasma is a complex combination of carbohydrates, proteins, minerals, vitamins and enzymes. It is a fluid that is mixe. Acta Vet Brno. 2019;88(4):377-84. https://doi.org/10.2754/avb201988040377.
Wu S, Shao Y, Zhang H, Wang S, Ji C, Zeng S, Ye H, Yu J. The effects of egg yolk concentration and particle size on donkey semen preservation. J Equine Vet Sci. 2018;65:19-24. https://doi.org/10.1016/j.jevs.2018.03.002.
Downloads
Publicado
Edição
Seção
Licença
Copyright (c) 2021 Brazilian Journal of Veterinary Research and Animal Science
Este trabalho está licenciado sob uma licença Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
O conteúdo do periódico está licenciado sob uma Licença Creative Commons BY-NC-SA (resumo da licença: https://creativecommons.org/licenses/by-nc-sa/4.0 | texto completo da licença: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode). Esta licença permite que outros remixem, adaptem e criem a partir do seu trabalho para fins não comerciais, desde que atribuam ao autor o devido crédito e que licenciem as novas criações sob termos idênticos.
