Detecção do gene mecA e resistência a diversos antibióticos em Staphylococcus aureus isolados do ouvido de gatos em um hospital localizado em Surabaya – Indonesia

Autores

  • Sergius Erikson Kaben Universitas Airlangga, Faculty of Veterinary Medicine, Profession Program in Veterinary Medicine
  • Mohammad Anam Al-Arif Universitas Airlangga, Faculty of Veterinary Medicine, Division of Veterinary Animal Husbandry
  • Anwar Ma’ruf Universitas Airlangga, Faculty of Veterinary Medicine, Division of Basic Veterinary Science
  • Mustofa Helmi Effendi Universitas Airlangga, Faculty of Veterinary Medicine, Division of Veterinary Public Health https://orcid.org/0000-0001-9727-411X
  • Shendy Canadya Kurniawan Wageningen University and Research, Department of Animal Sciences, Master Program of Animal Sciences, Specialisation in Molecule, Cell and Organ Functioning
  • Katty Hendriana Priscilia Riwu Universitas Mandalika, Faculty of Veterinary Medicine, Department of Veterinary Microbiology
  • Daniah Ashri Afnani Universitas Airlangga, Faculty of Veterinary Medicine, Master Program in Veterinary Disease and Public Health
  • Otto Sahat Martua Silaen Universitas Indonesia, Faculty of Medicine, Doctoral Program in Biomedical Science
  • Saumi Kirey Millannia Universitas Airlangga, Faculty of Veterinary Medicine, Profession Program in Veterinary Medicine
  • Safira Ramadhani Universitas Airlangga, Faculty of Veterinary Medicine, Profession Program in Veterinary Medicine
  • Agus Widodo Universitas Airlangga, Faculty of Vocational Studies, Department of Health
  • Thoriq Ihza Farizqi Universitas Airlangga, Faculty of Veterinary Medicine, Profession Program in Veterinary Medicine
  • Aswin Rafif Khairullah Universitas Airlangga, Faculty of Veterinary Medicine, Division of Veterinary Animal Husbandry

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2024.209027

Palavras-chave:

Staphylococcus aureus, Resistência múltipla a drogas, Gatos, Saúde pública

Resumo

Gatos são suscetíveis a adquirir S.aureus que colonizam principalmente as narinas e os ouvidos de espécies de felinos. A otite externa no ouvido dos gatos é uma das doenças produzidas pelo S.aureus nos animais. A terapia com antibióticos é o tratamento convencional para as infecções produzidas pelo S.aureus. Os antibióticos utilizados durante o prolongado tratamento e o emprego de sub doses podem selecionar microorganismos resistentes. Com base em tais argumentos torna-se necessária a pesquisa de S.aureus isolados do ouvido dos gatos, bem como, a realização de testes para a resistência a antibióticos e do gene mecA. Empregando swabs estéreis de algodão foram obtidas amostras dos ouvidos dos gatos em meio de Amies. O isolamento bacteriano foi efetuado em meio MAS e os testes catalase e coagulase foram realizados para a identificação das bactérias. A sensibilidade dos isolados de S.aureus foi avaliada com o emprego de discos dos antibióticos cefoxitin, tetraxiclina, eritromicina, gentamicina e cloranfenicol, incorporados no meio MHA. Todos os isolados positivos de S.aureus foram submetidos ao test MRSA para a detecção do gene mecA. A amostra investigada revelou 91% (91/100) de positivos para S.aureus, dos quais, 3,30% (3/91) foram resistentes a múltiplas drogas (MDR) pois foram resistentes a 3-4 classes de antibióticos. De 12 MRSA isolados analisados o gene mecA foi detectado em um isolado. O uso inapropriado de antibióticos é a causa da resistência bacteriana em pets. Adicionalmente o emprego excessivo de antibióticos em uma população pode resultar no desenvolvimento de resistência bacteriana adquirida a antibióticos. O uso de antibióticos em animais deve ser ordenado por uma administração de medicamentos apropriada para prevenir o desenvolvimento da resistência. 

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2024-04-12

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Kaben SE, Al-Arif MA, Ma’ruf A, Effendi MH, Kurniawan SC, Riwu KHP, et al. Detecção do gene mecA e resistência a diversos antibióticos em Staphylococcus aureus isolados do ouvido de gatos em um hospital localizado em Surabaya – Indonesia. Braz. J. Vet. Res. Anim. Sci. [Internet]. 12º de abril de 2024 [citado 16º de maio de 2024];61:e209027. Disponível em: https://www.journals.usp.br/bjvras/article/view/209027