Pitfalls in DNA extraction from ancient bones found in Brazilian shell-mounds
DOI:
https://doi.org/10.11606/issn.2448-1750.revmae.2007.89814Keywords:
Shell mounds, Ancient bones, DNA extraction of ancient bones, PCR of ancient DNAAbstract
The authors report their difficulties in DNA extraction from ancient bones found in shell-mounds of Sta. Catarina Island, SC, Brazil. The presence of humic acid, fulvic acid, free oxidant radicals and the hydrolytic effect of wet environment degrade the DNA to 20 to 30 base pairs, what jeopardizes any further utilization. The humic acid and the fulvic acid chelate magnesium ion necessary to Taq-polymerase activity, inhibiting the polymerase chain reaction (PCR). In this work the most efficient used protocols for DNA extraction, and the several PCR assay conditions are discussed.Downloads
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Published
2007-12-03
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Copyright (c) 2007 Daniela Gaeta Arruda, Mario Hideo Hirata, Orlando Cesar de Oliveira Barretto
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
ARRUDA, Daniela Gaeta; HIRATA, Mario Hideo; BARRETTO, Orlando Cesar de Oliveira. Pitfalls in DNA extraction from ancient bones found in Brazilian shell-mounds. Revista do Museu de Arqueologia e Etnologia, São Paulo, Brasil, n. 17, p. 461–467, 2007. DOI: 10.11606/issn.2448-1750.revmae.2007.89814. Disponível em: https://www.journals.usp.br/revmae/article/view/89814.. Acesso em: 19 may. 2024.